Inhibitory effect of oleanolic acid on non-enzymatic glycation and glycometabolism in insulin resistant HepG2 cells

Abstract In this paper, we studied the inhibitory effect of oleanolic acid (OA) on non-enzymatic glycosylation and improvement glycometabolism in insulin resistant (IR) human liver tumour (HepG2) cells. The anti-glycosylation activity OA was determined by bovine serum albumin (BSA) fructose model. results showed that moderately inhibited formation intermediates glycosylation, fructosamine ?-dicarbonyl compounds, strongly advanced glycation end products (AGEs). addition, analysed induced palmitic (PA) HepG2 had almost no impact cell viability at concentrations lower than 30 µM. With increase concentration, glucose production IR cells decreased, while glycogen content increased. Meanwhile, has a significant reactive oxygen species (ROS) levels IR-HepG2 Those suggested could be promising natural blood decreasing substance pharmaceutical functional food industries.